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KMID : 1134820080370111408
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Journal of the Korean Society of Food Science and Nutrition
2008 Volume.37 No. 11 p.1408 ~ p.1414
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Induction of Apoptosis by Scolopendra subspinipes mutilans in Human Leukemia HL-60 Cells through Bcl-xL Regulation
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Kim Kil-Nam
Kim Sang-Bum
Yoon Weon-Jong
Yang Kyoung-Sik
Park Soo-Yeong
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Abstract
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The anticancer properties of Scolopendra subspinipes mutilans extract were investigated. The extract from S. subspinipes mutilans by 80% EtOH was fractionated with n-hexane, dichloromethan (CH₂Cl₂), ethylacetate (EtOAc), and butanol (BuOH) in order. The EtOAc fraction showed the highest inhibitory activity (about 80%) against human leukemia (HL-60) cell growth at 50 ㎍/mL. To explore the mechanism of cytotoxicity, we used several measures of apoptosis to determine whether these processes were involved in EtOAc fraction-induced HL-60 cell death. Our results showed EtOAc fraction induced cell shrinkage, cell membrane blebbing, apoptotic body, and DNA fragmentation. The EtOAc fraction gradually decreased the expression of anti-apoptotic Bcl-xL and led to the activation of caspase-3, -9 and cleavage of PARP. These findings suggest that S. subspinipes mutilans exhibits potential anticancer properties.
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KEYWORD
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Scolopendra subspinipes mutilans, anticancer, cytotoxicity, sub-G1 hypodiploid cell, apoptosis
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